http://labmol.redirectme.net/zika/wp-content/uploads/sites/5/2016/10/urina-520x293.jpg 293 520 melina http://labmol.redirectme.net/zika/wp-content/uploads/sites/5/2016/05/openika-novo-1-300x227.png melina2016-10-11 15:05:052016-10-11 15:05:05Rapid molecular detection of Zika virus in urine using the recombinase polymerase amplification assay
Rapid molecular detection of Zika virus in urine using the recombinase polymerase amplification assay
A rapid point-of-care test is needed to detect the virus, especially at low resource settings. Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92% while the specificity was 100%. Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).
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